In the Paired-End SamplPrep_Guide_1005063_B protocol, there is a QC step to check the recovered DNA to ensure the presence of at least 0.5ug of fragmented DNA before performing end repair. When I follow old protocol, there is no such step, I usually check DNA using nanodrop after nebulization, never recover over 500ng of fragment DNA, but my library works fine so far. I am curios if any one has problem making libraries with less than 0.5ug of fragment DNA? thanks!
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