Hi all, when ligating adaptors and indexes, I have tried to amplify multiplexes from the 1st round of PCR in MASTR Dx BRCA assay using Q5 polymerase (ie, I left out completely Taq polymerase and something Multiplicom calles "universal PCR mix"). the products are longer (in yellow, please see the link), my only question is, should I be worried about the short bands around 120bp (presumably some nasty dimers)? Have you ever seen it in your reactions? thanx in advance for any suggestions..
-
Last edited by jana.supikova; 01-30-2014, 07:21 AM.
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...10-30-2026, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM