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Hi all, when ligating adaptors and indexes, I have tried to amplify multiplexes from the 1st round of PCR in MASTR Dx BRCA assay using Q5 polymerase (ie, I left out completely Taq polymerase and something Multiplicom calles "universal PCR mix"). the products are longer (in yellow, please see the link), my only question is, should I be worried about the short bands around 120bp (presumably some nasty dimers)? Have you ever seen it in your reactions? thanx in advance for any suggestions..
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Last edited by jana.supikova; 01-30-2014, 07:21 AM.
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