Library prep MiSeq

[Rüdiger]

Dear all,

I intend to send some gDNA samples to 16S library prep for Illumina MiSeq. I'm not doing it myself, so I just want to make sure that I do everything the most accurate way I can.

How much genomic DNA material the library prep requires?. Some of my samples have very low DNA concentrations, due to our sample origin. Some of them are OK (i.e. 24 ng/μL) but the lowest is 0.2 ng/μL. I have up to 40 μL of the sample, but I'm not sure of how much volume I should send.

Any recommendations that could help our provider prepare the libraries?

Thanks in advance!

[GenoMax]

This thread has some useful suggestions including a link to a protocol available from Illumina for this application (you may need to register for a free account): http://seqanswers.com/forums/showthread.php?t=39707 (Post #3)

[microgirl123]

These are questions you should ask your provider! Facilities often have different ways of prepping libraries and different requirements.