Can you really see miRNAs on gels before PCR amplification

[fisher]

I am new to miRNAseq. We plan to do miRNAseq using small RNA prep V1.0 and I am wondering if we could really see miRNAs on gel after each ligation?

Thanks a lot.

[kwaraska]

miRNA

I have never been able to see them. I just do a blind cut and pray a lot!

[fisher]

Thank you!

I finally cut it blindly too. But it worked!

I have never been able to see them. I just do a blind cut and pray a lot!

[link1]

if you use an adenylated 3' adapter you don't have to perform the small RNA gels and cuts. the adapter specifically will bind to your smalls.