How many samples can I run in a multiplex to get 'enough' reads per sample with an amplicon size of about 300 bp? Is there a formula to calculate this based on sequences generated per run, amplicon size and coverage?
Seqanswers Leaderboard Ad
Collapse
Announcement
Collapse
No announcement yet.
X
-
Here is a coverage calculator from Illumina: http://support.illumina.com/download...alculator.ilmn There is a tech note included for the coverage calculation.
An application note for 16s sequencing: http://res.illumina.com/documents/pr..._miseq_16s.pdf
I will let someone else comment on what qualifies as "enough" reads.
-
Originally posted by wafi View PostHow many samples can I run in a multiplex to get 'enough' reads per sample with an amplicon size of about 300 bp? Is there a formula to calculate this based on sequences generated per run, amplicon size and coverage?
here's the protocol: http://www.earthmicrobiome.org/emp-s...protocols/16s/
Comment
-
You can use the Coverage/Read calculator here: https://genohub.com/shop-by-next-gen...e7a8918f19251a I entered 960 samples at 30,000 reads/sample. Feel free to change those to fit your experiment.
- GenohubLast edited by Genohub; 04-22-2014, 02:28 PM.
Comment
Latest Articles
Collapse
-
by seqadmin
Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...-
Channel: Articles
04-04-2024, 04:25 PM -
-
by seqadmin
Despite advancements in sequencing platforms and related sample preparation technologies, certain sample types continue to present significant challenges that can compromise sequencing results. Pedro Echave, Senior Manager of the Global Business Segment at Revvity, explained that the success of a sequencing experiment ultimately depends on the amount and integrity of the nucleic acid template (RNA or DNA) obtained from a sample. “The better the quality of the nucleic acid isolated...-
Channel: Articles
03-22-2024, 06:39 AM -
ad_right_rmr
Collapse
News
Collapse
Topics | Statistics | Last Post | ||
---|---|---|---|---|
Started by seqadmin, 04-11-2024, 12:08 PM
|
0 responses
24 views
0 likes
|
Last Post
by seqadmin
04-11-2024, 12:08 PM
|
||
Started by seqadmin, 04-10-2024, 10:19 PM
|
0 responses
25 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 10:19 PM
|
||
Started by seqadmin, 04-10-2024, 09:21 AM
|
0 responses
22 views
0 likes
|
Last Post
by seqadmin
04-10-2024, 09:21 AM
|
||
Started by seqadmin, 04-04-2024, 09:00 AM
|
0 responses
52 views
0 likes
|
Last Post
by seqadmin
04-04-2024, 09:00 AM
|
Comment