How many samples can I run in a multiplex to get 'enough' reads per sample with an amplicon size of about 300 bp? Is there a formula to calculate this based on sequences generated per run, amplicon size and coverage?
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Here is a coverage calculator from Illumina: http://support.illumina.com/download...alculator.ilmn There is a tech note included for the coverage calculation.
An application note for 16s sequencing: http://res.illumina.com/documents/pr..._miseq_16s.pdf
I will let someone else comment on what qualifies as "enough" reads.
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Originally posted by wafi View PostHow many samples can I run in a multiplex to get 'enough' reads per sample with an amplicon size of about 300 bp? Is there a formula to calculate this based on sequences generated per run, amplicon size and coverage?
here's the protocol: http://www.earthmicrobiome.org/emp-s...protocols/16s/
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You can use the Coverage/Read calculator here: https://genohub.com/shop-by-next-gen...e7a8918f19251a I entered 960 samples at 30,000 reads/sample. Feel free to change those to fit your experiment.
- GenohubLast edited by Genohub; 04-22-2014, 02:28 PM.
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