Our lab is having problems getting successful dual index runs on the HiSeq 2500 rapid module (upgraded from the HiSeq 2000). Single index runs work, but on dual index runs the quality craps out before it can read the second index.
Illumina told us that it could be a template diversity issue, but spiking in PhiX up to 30% did not resolve the issue.
I am wondering if this is specific to our HiSeq, or if other labs have noticed similar problems.
Thanks,
Brett
Illumina told us that it could be a template diversity issue, but spiking in PhiX up to 30% did not resolve the issue.
I am wondering if this is specific to our HiSeq, or if other labs have noticed similar problems.
Thanks,
Brett
Comment