Originally posted by GW_OK
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what exactly do you mean by reuse agilent sureselect? if you are referring to the capture library, then that is a bad idea. the streptavidin beads will pull down all of the biotinylated RNA "baits" whether complementary genomic sequence has been captured or not.
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I suspect the "bait" RNA is digested during the bead wash steps. Also, Agilent furtively releases updates to the sureselect protocol, so make sure you are following most recent, http://www.opengenomics.com/sureselect
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