My lab is just starting up with the Peterson et al. (2011) protocol. I am testing restriction enzymes now and about to run the samples on a TapeStation.
The next step is to order the oligos for the adaptors/primers. Is there any thing I need to know about ordering the adaptors/primers or can I just order standard desalted oligos from IDT just like I do for Sanger sequencing?
The next step is to order the oligos for the adaptors/primers. Is there any thing I need to know about ordering the adaptors/primers or can I just order standard desalted oligos from IDT just like I do for Sanger sequencing?
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