Do you think it's sequencing error?

[mingkunli]

TAT-TTTCGTCTG
TATCTTTCGTTTG


These two kinds of reads appear several times, also TAT-TTTCGTTTG.
I know it's likely to be sequencing error for 454 data, but I don't know any mechanism can be responsible for this phenomenon with solexa sequencing.

What's your opinion?

[lh3]

Due to amplification or misalignment. So far as I know, we do not think this is sequencing error.

[Torst]

TAT-TTTCGTCTG
TATCTTTCGTTTG
These two kinds of reads appear several times, also TAT-TTTCGTTTG.
I know it's likely to be sequencing error for 454 data, but I don't know any mechanism can be responsible for this phenomenon with solexa sequencing.

Try searching for that sequence in the list of Illumina primers and adaptors: http://seqanswers.com/forums/showthread.php?t=198

[mingkunli]

Try searching for that sequence in the list of Illumina primers and adaptors: http://seqanswers.com/forums/showthread.php?t=198

Thanks, seems it's not there.