mRNA-seq library quality control

[shilh]

Hi everyone,

First of all, I am so glad that I could find a forum that we can discuss NGS related issues.

My experiment is to detect gene expression profile after my target protein knockdown.

here, my question is: how everyone check his/her mRNA-seq library quality after library construction in addition to bioanalyzer and real time PCR?

Best
lihong

[skycreative]

usually, we do not check the library , after cluster growth, just sequencing and analysis the data.
If you really want to know the features of your library, maybe you can do TA cloning before amplify the library. then pick the clone and sequencing part of plasmid using Sanger sequence method

[shilh]

Thanks!

I see. I will think about that after I get my bioanalyzer results.