[olesk]

Well, time has passed since the original post. We have just submitted a ms were we found 8 suppressing mutations, one in each of 8 E. coli mutants, as well as some shared mutations in the background strain. The mutations included everything from the "mutation-zoo": Chr. rearrangements, IS elements jumping, SNP's, simple indels and indels between repeats. Everything was found from single end reads with R2R: http://milne.ruc.dk/R2R/
Ask for more info if needed,

[james hadfield]

Hi,

A bit late to comment but I would have suggested longer paired end reads as the most cost effectve way to get genome coverage. On a per bp basis PE100 trumps PE36 by some way.

James.