is it normal to have a Read 1 and Read 2 each with 32 million reads in a RNASeq sample from a patient? This is from a MiSeq run.
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32 M read output from a MiSeq run is higher than specification and most likely quality will be low. -
so what is the usual output? also, what is the correlation between read quality and number of reads? thank you.Comment
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MiSeq tech specs can be found here: http://systems.illumina.com/systems/...fications.html
General wisdom is that having an overclustered flowcell leads to drops in Q scores (especially later in a the run). That said, YMMV.Last edited by GenoMax; 11-06-2014, 04:00 AM.Comment
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