Anyone running 16S metagenomic studies on a MiSeq who can give me an average cost estimate per samples to run several hundred samples?
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We run an adaptation of the Illumina-demonstrated protocol and it costs ~$12 per sample, exclusive of the MiSeq run. -
Where can I find the doc of this demonstrated protocol? This one?Originally posted by microgirl123 View Post
$12/sample means each sample has about 100,000 overlapping PE300 reads?Comment
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NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
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