I've just had a very weird MiSeq run pop up, and there are really several things that could be wrong with it - low diversity library, V2 kit (expired), and likely drastically underloaded. The pass filter rate was just terrible. I've never seen the Q score trace look like this, though. Does anyone have any ideas? I'm just interested in getting a diagnosis at this point....
-
-
Given there's a bunch of bases that start with low Q-scores and continue with low Q-scores, I wonder if there's a particular tile on the flow cell that was dirty or otherwise gave bad data. On Basespace/SAV I'd go to the Flowcell Chart and select "Median Q-Score" and look at a handful of cycles to see if certain tiles are consistently giving lousy Q-scores. Also, don't forget to select 'Both Surfaces' (unless you used a nano flow cell in which case you only image one surface).
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment