Non HPLC purified primers for library preparation?

[TVC]

Non HPLC purified primers for library preparation?

Hi,

We are planning to perform library preparation for Illumina seq but because of the large number of samples and primers to be analysed, the cost of HPLC purification for so many primers is quite substantial. How important is it to use HPLC purified primers compared to standard desalted primers in the library preparation PCRs?

Has anyone got any experiences with this?

Thanks a lot!

[Gonza]

Hi,

You can get standard desalted primers and have the same results as if you'd order HPLC purified ones. This will help:

http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0094651

"...Finally, we report a modification to the library preparation protocol whereby cDNA samples were bar-coded with non-HPLC purified primers, without affecting the quality and quantity of the RNA-seq data...."