Hello community,
Firstly I would like to greet everyone, for this is my first of hopefully many productive experiences on this forum.
My question involves amplification free library preparation for MiSeq. We have purified aptamers of 80 bp and would like to prepare them for sequencing with as little amplification as possible.
Is it possible to directly ligate indexes to aptamers are do we have to ligate adapters first?
Is there any other way?
Thank you in advance for any suggestions.
Firstly I would like to greet everyone, for this is my first of hopefully many productive experiences on this forum.
My question involves amplification free library preparation for MiSeq. We have purified aptamers of 80 bp and would like to prepare them for sequencing with as little amplification as possible.
Is it possible to directly ligate indexes to aptamers are do we have to ligate adapters first?
Is there any other way?
Thank you in advance for any suggestions.
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