Throughput of Truseq SBS v2 kit??

[ymc]

I could find v1 and v3 but not v2. Anyone knows?

Thanks in advance!

HiSeq 2000 v3 dual flowcell 8-lane/flowcell
------------------------------------------------
1x35 2days 95-105Gbp 2.7-3B 90%Q30
2x50 5.5days 270-300Gbp 5.4-6B 85%Q30
2x100 11days 540-600Gbp 5.4-6B 80%Q30

HiSeq 2000 v1 dual flowcell 8-lane/flowcell
------------------------------------------------
1x35 1.5days 26-35Gbp 750M-1B 90%Q30
2x50 4days 75-100Gbp 1.5-2B 90%Q30
2x100 8days 150-200Gbp 1.5-2B 85%Q30

[ymc]

Found this Illumina ppt claiming it goes from 250Gbp to 600Gbp:

http://gqinnovationcenter.com/documents/rendezvous/8-betley.pdf

Perhaps, v2 looks like this?

HiSeq 2000 v2 dual flowcell 8-lane/flowcell
------------------------------------------------
1x35 1.5days 33-44Gbp 940M-1.25B 90%Q30
2x50 4days 94-125Gbp 1.88-2.5B 90%Q30
2x100 8days 188-250Gbp 1.88-2.5B 85%Q30

[pmiguel]

I don't remember there being a v2. Chemistry seemed to jump from v1 to v3 for highoutput HiSeq (8-lane flow cell) chemistry.

--
Phillip

[ymc]

I don't remember there being a v2. Chemistry seemed to jump from v1 to v3 for highoutput HiSeq (8-lane flow cell) chemistry.

--
Phillip

Another piece of evidence that supports the existence of v2

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679043/

[Jessica D.]

It's been long enough that I've forgotten most of the pre-v3 information that I used to know, and the notebooks are all in storage at this point.

I did find this:
http://wenku.baidu.com/view/1d7bde54...a50e27071.html

which reminded me: the cluster generation kits went through a couple of iterations between SBS sequencing v1 and v3. IIRC, there was such a thing as a v2 cluster kit, that worked with the v1 sequencing reagents. At the time v3 SBS reagents came out for the HiSeq, the cluster kits went to v3 since the increase in sequencing output was at least partly due to some flow cell changes.

[ymc]

Another paper used the SBS v2 kit:

http://www.clinchem.org/content/earl...96014.full.pdf

[pmiguel]

Another piece of evidence that supports the existence of v2

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3679043/

Yes, given the evidence you provide, I guess there was HiSeq v2 SBS chemistry. The only time we used v1 chemistry was during the install run on our instrument. For an install run, Illumina must have just been using up there stores of v1 chemistry at that time.

--
Phillip