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  • 5 microgram for Illumina

    Dear All,

    I have only 5 microgram in my sample, is that enough for Illumina sequencing?

    Thanks a lot!

    Wei

  • #2
    DNA or RNA? In either case it should be fine.

    Comment


    • #3
      Thanks shurjo!

      They are total RNA sample. Because in the protocol, 1-2 ug was used as the starting material. But the people in core facility told me it is better give them more, out last sample for sequencing was around 7 ug. Therefore, just ask experienced RNA-Sequencers if 5 ug is fine.

      Nice weekend!
      Wei

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      • #4
        I just did a RNA prep using NEB reagents and QIAGEN spin columns using ~900ng total RNA and it was fine.. Your 5ug will be perfect.

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        • #5
          Thanks peromhc!

          I will just send this sample for sequencing.

          Comment

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