A C G T intensities

[suludana]

Hi,
I made an Solexa experiment where the confidence level of "A" was too low. Why this happened? There is a way to avoid this?

Thanks

[new300]

Could you post one of the IVC plots? (the intensity plots you get from the Summary.htm page). Is this a GA1 or GA2?

[suludana]

A C G T intensities

Hi,
the intensity values obtained were:

A - 3000
C - 4000
G - 6000
T - 19000

Thanks for your interest.

Suludana

[suludana]

A C G T intensities

It´s a GA I.

Thanks

[new300]

Those intensities seem very weird to me. Could you post one of the plots for a bad lane e.g.: s_1_all.png

[suludana]

Hi,
right now I do not have the file you ask me.
I'm talking about the values given in the first-cycle report.

A intensity: 3,000
C intensity: 4,000
G intensity: 6,000
T intensity: 19,000

[new300]

hmm.. It's difficult to without the full plot but something seems very wrong to me, the T intensity should diverge so much.