Hi All,
I cannot resist asking the community about its feeling/experience on the underrated issue we've been studying (open access: http://nar.oxfordjournals.org/conten.../18/nar.gkv107).
It's surprising that loads of cross-contaminations created by the switching of tags/indices sequences appended to amplification primers and used for sample demultiplexing aren't addressed much... Did you noticed this in your metagenomic amplicon datasets?
Cheers to all!
I cannot resist asking the community about its feeling/experience on the underrated issue we've been studying (open access: http://nar.oxfordjournals.org/conten.../18/nar.gkv107).
It's surprising that loads of cross-contaminations created by the switching of tags/indices sequences appended to amplification primers and used for sample demultiplexing aren't addressed much... Did you noticed this in your metagenomic amplicon datasets?
Cheers to all!
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