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^ I have not but this was something that was just suggested to me and I might give it a try. For generating the matrix file, how many tiles do you think is adequate? And do you do all cycles or can I shorten that as well?
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I usually pick 10-20 tiles and then run for 25 cycles. I try to avoid picking tiles from the flowcell edges as they're more prone to have problems. It runs pretty fast and gives you a Bustard.xml that you can check it to see if the pass filter/etc looks reasonable before doing a full pipeline.Comment
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Does adding the PhiX spike-in to each sample have the same effect as adding an ERCC control? Ambion offers this ERCC control to help with accurately quantify transcript levels, but I'm not sure if I need to invest in this addition or not... Any thoughts?
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Technological advances have led to drastic improvements in the field of precision medicine, enabling more personalized approaches to treatment. This article explores four leading groups that are overcoming many of the challenges of genomic profiling and precision medicine through their innovative platforms and technologies.
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM
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