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^ I have not but this was something that was just suggested to me and I might give it a try. For generating the matrix file, how many tiles do you think is adequate? And do you do all cycles or can I shorten that as well?
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I usually pick 10-20 tiles and then run for 25 cycles. I try to avoid picking tiles from the flowcell edges as they're more prone to have problems. It runs pretty fast and gives you a Bustard.xml that you can check it to see if the pass filter/etc looks reasonable before doing a full pipeline.Comment
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Does adding the PhiX spike-in to each sample have the same effect as adding an ERCC control? Ambion offers this ERCC control to help with accurately quantify transcript levels, but I'm not sure if I need to invest in this addition or not... Any thoughts?
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The sequencing world is rapidly changing due to declining costs, enhanced accuracies, and the advent of newer, cutting-edge instruments. Equally important to these developments are improvements in sequencing analysis, a process that converts vast amounts of raw data into a comprehensible and meaningful form. This complex task requires expertise and the right analysis tools. In this article, we highlight the progress and innovation in sequencing analysis by reviewing several of the...05-06-2024, 07:48 AM -
The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...04-22-2024, 07:01 AM
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