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  • jo_mason
    Member
    • Dec 2010
    • 20

    #16
    Can anyone tell me how long it took for Illumina to commision your HiSeq after delivery. I've just had a timetable sent to me which takes a little over two months and that doesn't include the training!
    Jo Mason

    [email protected]

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    • NextGenSeq
      Senior Member
      • Apr 2009
      • 482

      #17
      We've been waiting for six months and it's still not here (a month after it's scheduled delivery).

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      • Lee Sam
        Member
        • Oct 2008
        • 57

        #18
        Originally posted by juan View Post
        200 million reads per lane? Is that the standard or are you pushing the limit? What is the standard # of reads/lane for a HiSeq?
        Is the RTA of the HiSeq the same as GAII? In a GAII run the low-quality ends of reads are flagged with a quality score of 2 (ASCII B). Does the HiSeq RTA also output reads in this manner? What % of reads in the first and second run of a pair are low quality (QV=2)?
        Is it comparable to GAII (<15% in first run, up to 40% in second run).
        Sorry, I should have been more clear. 100 million paired reads (or 200 million ends).

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