Does anyone have any suggestions about how to filter out the mis-called variants on Nextseq? Also, has anyone used the v2 kits? Is the fidelity significantly improved?
-
-
We are about to run a test on the NextSeq v2 chemistry, so I'll probably have some data in a week or two. -
Thank you. We are anxious to see if we can improve the reliability of our Nextseq data. Our group at the National Cancer Institute is attempting to identify mutated gene products expressed in cancer cells that can be recognized by patient immune cells that will allow us to develop effective cancer therapies. We purchased a Nextseq because we needed to get our data quickly in order to develop treatments for patients with advanced cancer, and we have already identified many targets using this approach. Enhancing the accuracy of the variant calls would make this process simpler, as it would help to eliminate many of the false positives. Some of these are easy to identify as they appear in repeated tumor samples - I presume these are regions of the genome that are difficult to sequence. We also merge whole exome with RNAseq, which helps to validate the calls.Comment
-
CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM -
Proteomics platforms are evolving rapidly, with advances in mass spectrometry and affinity-based approaches expanding what researchers can detect and at what scale. As the field moves toward deeper proteome coverage and clinical applications, scientists face an increasingly complex landscape of tools. This article will explore how researchers are navigating these choices to find the right platform for their work.
The systematic characterization of the human proteome has...07-20-2026, 11:48 AM
Comment