Dear All,
We are doing resequencing of various bacteria, but it seems to be complicated to do Mycobacteria sequencing, regardless if its M. tuberculosis or non tuberculous Mycobacteria.
DNA Extraction was done after cooking the samples for 10 Minutes using either Qiagen or MagnaPure Compact.
For the Library preparation and sequencing nextera Kits were used.
Results were awful, just a few reads
Maybe some of you can give us some advice
THX
Alexander
We are doing resequencing of various bacteria, but it seems to be complicated to do Mycobacteria sequencing, regardless if its M. tuberculosis or non tuberculous Mycobacteria.
DNA Extraction was done after cooking the samples for 10 Minutes using either Qiagen or MagnaPure Compact.
For the Library preparation and sequencing nextera Kits were used.
Results were awful, just a few reads
Maybe some of you can give us some advice
THX
Alexander
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