Hi,
I will sequence a gene and I need to decide betwen single or paired sequencing.
What kind of criterion should I take? ... I know I must use paired-end if I want to read a G rich sequence or if I work with large rearrangements or delections... What else? Any other important thing?
Thanks
I will sequence a gene and I need to decide betwen single or paired sequencing.
What kind of criterion should I take? ... I know I must use paired-end if I want to read a G rich sequence or if I work with large rearrangements or delections... What else? Any other important thing?
Thanks
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