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  • KemKa
    Junior Member
    • Apr 2015
    • 1

    #1

    Biallelic SNPs in Tetraploids via the UNEAK pipeline

    Hello,

    currently I am working on a supposed autotetraploid plant without a reference genome. We got data from Illumina sequencing and did SNP calling via the Tassel UNEAK pipeline.
    Now I come to my question, why are all the SNPs we got from the pipeline bi-allelic? I would suppose that I could also have triallelic or even tetraallelic SNPs in a tetraploid plant. Maybe it`s a stupid question but I`m new in this field and I`m the only one in our group working on tetraploids.

    I would be glad if somebody could answer my question
    Thank you in advance
  • luc
    Senior Member
    • Dec 2010
    • 469

    #2
    I am not familiar with Tassel; I assume though that triallelelic SNPs are so rare that they do not warrant the effort to accommodate them bioinformaticaly.

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