Hi everyone,
We accidentally thawed the wrong type of reagent cartrige for loading samples onto our Illumina MiSeq machine. We noticed only after the cartrige was completely thawed, that it contained reagents for only 152 cycles, whereas we needed 600 cycles.
Of course we do not want to throw the reagent cartridge away, so we opted to refreeze it for now.
I know that Illumina does not recommend using refrozen reagent cartridges, but I do not see why it should not work...
Does anybody have any experience with MiSeq sequencing runs using a reagent cartridge that was already thawed once and then refrozen?
Or does anybody know specific reasons why one should not try to use refrozen reagent cartridges?
We accidentally thawed the wrong type of reagent cartrige for loading samples onto our Illumina MiSeq machine. We noticed only after the cartrige was completely thawed, that it contained reagents for only 152 cycles, whereas we needed 600 cycles.
Of course we do not want to throw the reagent cartridge away, so we opted to refreeze it for now.
I know that Illumina does not recommend using refrozen reagent cartridges, but I do not see why it should not work...
Does anybody have any experience with MiSeq sequencing runs using a reagent cartridge that was already thawed once and then refrozen?
Or does anybody know specific reasons why one should not try to use refrozen reagent cartridges?
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