Dear all
One of our projects needs PE50 sequencing.
With Illumine HiSeq 1500, one Rapid Run PE50 has been accomplished, but per sample sequencing data (~60% of total) is not sufficient. We are thinking to have more sequencing reads (~40% of total) by using High Output run PE100 then trim these reads to PE50 ones.
Is there any concern for combining such two types of PE50 sequencing reads together? Both RR and HO runs will share the same file setting as zipping output bcl files and bin Q scores.
Thank you thank you.
Wei
One of our projects needs PE50 sequencing.
With Illumine HiSeq 1500, one Rapid Run PE50 has been accomplished, but per sample sequencing data (~60% of total) is not sufficient. We are thinking to have more sequencing reads (~40% of total) by using High Output run PE100 then trim these reads to PE50 ones.
Is there any concern for combining such two types of PE50 sequencing reads together? Both RR and HO runs will share the same file setting as zipping output bcl files and bin Q scores.
Thank you thank you.
Wei
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