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  • BHR
    Junior Member
    • Jul 2015
    • 4
    #16
    Originally posted by Kurt Lamour View Post
    Just to finish this one up - after talking to KAPA and trying lots of different things - the thing that worked was to re-anneal my adapters. I did this by ramping slowly (0.1C/sec) from 95C (incubate 5 min) to 4C. fixed everything. Appreciate thoughts on site!
    Sorry, are you referring to adapter ligation step? isn't 95c 5' killing the ligase?

    I asked because I also found the efficiency of ligation is low for PCR amplicons for us.

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