First HiSeq 3000 data

[DNATECH]

Yes, an average of 340 million clusters passing filter per lane.

You mean 340 million pass-filter clusters per lane?

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Phillip

[pmiguel]

What final concentration was the phiX library that you clustered? I mean after neutralization?

I mean is there no danger of overclustering anymore? That was what I was hoping for when I heard about the patterned flowcells...

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Phillip

[pmiguel]

Hello,

in case you are interested, we have posted some results from our first HiSeq 3000 runs as well as some information and considerations on the changes introduced by the new HiSeq 3000 and Hiseq 4000 sequencer generation. The yields on the first two runs were higher than expected at about 340 million reads per lane.

You mean 340 million pass-filter clusters per lane?

--
Phillip

[GenoMax]

@DNATECH: Based on this (and your other post) it sounds like you need "near perfect libraries" to get good data from patterned flowcells. This could be a problem for core facilities, where "variable" quality libraries come in from customers.

It would be interesting to hear about your experiences as real world customer libraries start flowing through.

[DNATECH]

We are actually waiting for flow-cells for several weeks; I guess Illumina is surprised that anybody wants to use the new sequencers?

[idedios]

Sweet my company just got one and we'll do our training run in a couple weeks from now. The instrument was setup and configured nearly a month ago so it's bothering to see it idle for so long.

[DNATECH]

Some Q30 plots.

[DNATECH]

Hello,

in case you are interested, we have posted some results from our first HiSeq 3000 runs as well as some information and considerations on the changes introduced by the new HiSeq 3000 and Hiseq 4000 sequencer generation. The yields on the first two runs were higher than expected at about 340 million reads per lane. The quality looks good.
There is also link to the complete data from a PhiX lane (including the clusters that did not "pass filter").

http://dnatech.genomecenter.ucdavis.edu/2015/05/07/first-hiseq-3000-data-download/

Btw, the cluster images do not give away the patterned character of the flowcells. Please see the attachment.

Best,
Lutz