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  • DNATECH
    replied
    Yes, an average of 340 million clusters passing filter per lane.

    Originally posted by pmiguel View Post
    You mean 340 million pass-filter clusters per lane?

    --
    Phillip

    Leave a comment:


  • pmiguel
    replied
    What final concentration was the phiX library that you clustered? I mean after neutralization?

    I mean is there no danger of overclustering anymore? That was what I was hoping for when I heard about the patterned flowcells...

    --
    Phillip

    Leave a comment:


  • pmiguel
    replied
    Originally posted by DNATECH View Post
    Hello,

    in case you are interested, we have posted some results from our first HiSeq 3000 runs as well as some information and considerations on the changes introduced by the new HiSeq 3000 and Hiseq 4000 sequencer generation. The yields on the first two runs were higher than expected at about 340 million reads per lane.
    You mean 340 million pass-filter clusters per lane?

    --
    Phillip

    Leave a comment:


  • GenoMax
    replied
    @DNATECH: Based on this (and your other post) it sounds like you need "near perfect libraries" to get good data from patterned flowcells. This could be a problem for core facilities, where "variable" quality libraries come in from customers.

    It would be interesting to hear about your experiences as real world customer libraries start flowing through.

    Leave a comment:


  • DNATECH
    replied
    We are actually waiting for flow-cells for several weeks; I guess Illumina is surprised that anybody wants to use the new sequencers?

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  • idedios
    replied
    Sweet my company just got one and we'll do our training run in a couple weeks from now. The instrument was setup and configured nearly a month ago so it's bothering to see it idle for so long.

    Leave a comment:


  • DNATECH
    replied
    Some Q30 plots.
    Attached Files

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  • DNATECH
    started a topic First HiSeq 3000 data

    First HiSeq 3000 data

    Hello,

    in case you are interested, we have posted some results from our first HiSeq 3000 runs as well as some information and considerations on the changes introduced by the new HiSeq 3000 and Hiseq 4000 sequencer generation. The yields on the first two runs were higher than expected at about 340 million reads per lane. The quality looks good.
    There is also link to the complete data from a PhiX lane (including the clusters that did not "pass filter").

    http://dnatech.genomecenter.ucdavis....data-download/

    Btw, the cluster images do not give away the patterned character of the flowcells. Please see the attachment.

    Best,
    Lutz
    Attached Files
    Last edited by DNATECH; 05-07-2015, 12:48 AM.
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