Originally posted by pmiguel
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What final concentration was the phiX library that you clustered? I mean after neutralization?
I mean is there no danger of overclustering anymore? That was what I was hoping for when I heard about the patterned flowcells...
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PhillipLeave a comment:
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@DNATECH: Based on this (and your other post) it sounds like you need "near perfect libraries" to get good data from patterned flowcells. This could be a problem for core facilities, where "variable" quality libraries come in from customers.
It would be interesting to hear about your experiences as real world customer libraries start flowing through.Leave a comment:
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We are actually waiting for flow-cells for several weeks; I guess Illumina is surprised that anybody wants to use the new sequencers?Leave a comment:
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Sweet my company just got one and we'll do our training run in a couple weeks from now. The instrument was setup and configured nearly a month ago so it's bothering to see it idle for so long.Leave a comment:
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First HiSeq 3000 data
Hello,
in case you are interested, we have posted some results from our first HiSeq 3000 runs as well as some information and considerations on the changes introduced by the new HiSeq 3000 and Hiseq 4000 sequencer generation. The yields on the first two runs were higher than expected at about 340 million reads per lane. The quality looks good.
There is also link to the complete data from a PhiX lane (including the clusters that did not "pass filter").
http://dnatech.genomecenter.ucdavis....data-download/
Btw, the cluster images do not give away the patterned character of the flowcells. Please see the attachment.
Best,
LutzLast edited by DNATECH; 05-07-2015, 12:48 AM.
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Amplicon sequencing is a targeted approach that allows researchers to investigate specific regions of the genome. This technique is routinely used in applications such as variant identification, clinical research, and infectious disease surveillance. The amplicon sequencing process begins by designing primers that flank the regions of interest. The DNA sequences are then amplified through PCR (typically multiplex PCR) to produce amplicons complementary to the targets. RNA targets...03-21-2023, 01:49 PM -
Targeted sequencing is an effective way to sequence and analyze specific genomic regions of interest. This method enables researchers to focus their efforts on their desired targets, as opposed to other methods like whole genome sequencing that involve the sequencing of total DNA. Utilizing targeted sequencing is an attractive option for many researchers because it is often faster, more cost-effective, and only generates applicable data. While there are many approaches...03-10-2023, 05:31 AM
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