I'm wondering if anyone has identified a good primer pair that spans the 18s rRNA v4 region and is proven to work well on the MiSeq platform. I haven't found any established protocols in the literature yet. Most of the recent publications from 2014 refer to 454 sequencing. I'd be interested in other primer suggestions spanning v5, v7 and v8 as well. I've found this paper useful, "Characterization of the 18S rRNA Gene for Designing Universal Eukaryote Specific Primers" by Hadziavdic et al., but again, all these primers were designed with 454 in mind.
If anyone is willing to share universal eukaryotic 18s primers and amplification protocols they have found to work well on the MiSeq, I'd be really appreciative.
If anyone is willing to share universal eukaryotic 18s primers and amplification protocols they have found to work well on the MiSeq, I'd be really appreciative.
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