Hi,
Has anyone ever had primers that were too concentrated for the paired-end sample prep? We are getting excess primers left over after the PCR, which is after the gel purification. But the excess primers were not there before the PCR. Moreover, our first batch of PE sample preps were fine (i.e. no excess primers).
PF
Has anyone ever had primers that were too concentrated for the paired-end sample prep? We are getting excess primers left over after the PCR, which is after the gel purification. But the excess primers were not there before the PCR. Moreover, our first batch of PE sample preps were fine (i.e. no excess primers).
PF
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