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  • NextSeq data bias

    Hi,
    We notice an unexpected variation of the estimated library size as a function of the number of reads, even after downsampling with NextSeq data. This is not observed with MiSeq-generated datasets. All analyses were done on Picard.

    Did anybody else duplicate this observation? Does this ring a bell?
    Thanks for your help,
    Kamran
    Kamran SHAZAND, PhD

  • #2
    If you are using V1 chemistry, that's probably caused by the high error rate. But could you explain a bit more about exactly what the variation was, and your methodology?

    Comment


    • #3
      Thanks Brian. We have moved to V2 but the analyzed data were from the V1 period.
      Is there an email where I can send you a slide?
      Kamran SHAZAND, PhD

      Comment


      • #4
        Originally posted by kshazand View Post
        Thanks Brian. We have moved to V2 but the analyzed data were from the V1 period.
        Is there an email where I can send you a slide?
        Sure - pm sent.

        Comment

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