I've been looking at doing some shotgun metagenomic sequencing on the HiSeq 2500 (or 3000/4000). It seems that with both Nextera DNA and Truseq Nano (or PCR-free) Illumina only has indexes that allow multiplexing up to 96 samples. I would, ideally, like to multiplex far beyond this, to 200 samples or more.
Does anyone know of a third party company that makes additional indexes for Truseq or Nextera DNA that will allow me to multiplex higher? Or has anyone made their own indexes?
Does anyone know of a third party company that makes additional indexes for Truseq or Nextera DNA that will allow me to multiplex higher? Or has anyone made their own indexes?
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