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  • microgirl123
    Senior Member
    • Jun 2012
    • 199
    #1

    Less than 2 nM for a MiSeq run?

    I'm sure this has been posted here before, but I can't find it!

    I've got some gDNA libraries that are coming in as less than 2 nM with qPCR quantification after PCR enrichment. I know there's a way to adjust the MiSeq denaturation/dilution/loading protocol to allow for a lower starting concentration. Does anyone do this or remember the thread this info was posted in?
    Tags: None
    • HESmith
      Senior Member
      • Oct 2009
      • 512
      #2
      http://seqanswers.com/forums/showthr...dilute+samples

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