I've never done RAD-seq before but I have the opportunity to do a MiSeq run. RAD-seq data would be most useful to me at this point (no current genomic information on my taxa), but I've not seen anyone use MiSeq for this purpose. I assume it's due to lack of sequence depth, but I wonder if there are other concerns as well. Has anyone used a MiSeq for RAD-seq? Are there other reasons why this would be a wasted effort?
If I push forward with RAD-seq analysis using the MiSeq, should I reduce the number of individuals or or modify protocols to somehow significantly reduce the number of fragments?
Any suggestions and/or discussion would be helpful. Thanks!
If I push forward with RAD-seq analysis using the MiSeq, should I reduce the number of individuals or or modify protocols to somehow significantly reduce the number of fragments?
Any suggestions and/or discussion would be helpful. Thanks!
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