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  • sunnycqcn
    Member
    • Apr 2013
    • 17

    #1

    Extract the header of illumina sequence!

    I have question about our DNA sequence data.
    If the format of sequence data is
    @HWI-ST1234:136:C5F6VACXX:6:1101:4121:2231 1:N:0:ACTTGA
    TATGGGTTTCCACGGAGCACAGTGCCTAGTGCTCACTCCCCAGTTGTATCTTATTTTTCAGGTCAGCAGGTCGGGCCGGGAGTGTGACATGACGGAGCAGA
    +
    CCCFFFDDHHHHHJJGIJJJJJHIJJIJJHIJIJIJJJJJJIIIIJGIIBFHHFHJJJG>FHIJIGIIEHAHBBAB@BDBDD<?ACA>CDDDDDD5<BBD?.
    I can extract the sequence identifier as @HWI-ST1234:136:C5F6VACXX:6:1101:4121:2231 1:N:0:ACTTGA using the code.
    If the format of sequence data is
    @HWI-ST1234:136:C5F6VACXX:6:1101:4295:2242 1:N:0:ACTTGA
    AATACTTGTACGAGGGTGTTTTGCCACACCATATCTCATAAGGTGTGTTGGGTACATCTTTACTTGTCATTCTATTCAAAATATGTGTTGTTGTTTC
    +
    @@@ADD?DH8FH1CGG2A<F@FH?@?FC1DFGEDB9?BFHHIF?8?DBC=FB5@CDA;@)=.))..).;;B@B?@>>BDCCCCCD>B;?=5??<?CC
    I can not extract the sequence identifier.

    So I think the problem is the sequence data. The first symbol of second one is @. The first symbol of identifier also is @. So the code can not extract the correct sequencing identifier from our sequence data.
    I want to extract the sequence identifier form my sequence data. The identifier format is @HWI-ST1234:136:C5F6VACXX:6:1101:4295:2242 1:N:0:ACTTGA. Could you help me do it?
    Thanks,
    Fuyou
  • Brian Bushnell
    Super Moderator
    • Jan 2014
    • 2709

    #2
    What do you want to do with the headers?

    Comment

    • GenoMax
      Senior Member
      • Feb 2008
      • 7142

      #3
      Since every fastq header is going to start with "@HWI-ST1234" you could check for the longer string rather than just "@". Or you could take advantage of the periodicity (every nth line will be a header) as long as your sequences don't warp around.

      Comment

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