Different flow cell ànd reagents for single-end and paired end sequencing (HiSeq)?

[GraceM]

Hi all,

I was wondering whether there are different reagents used for a single end versus a paired end sequencing run on HiSeq?
The following presentation (http://skatebase.org/sites/skatebase...lluminaNGS.pdf) nicely illustrates the difference in oligos when bound to a flow cell for single-end versus paired end sequencing (page 41-42). However, it was told to me that you can use the same reagents for both sequencing protocols but according to this ppt, either a chemical (periodate) or an enzyme is used for linearization of the P5 oligo before Read 1 primer binding suggesting different required reagents. Can anyone share some ideas about this?

Thanks a lot!

[HESmith]

The flow cells, reagents, and protocols all differ between single-end vs paired-end sequencing.