Curious to see how often other users are washing their MiSeqs. We are currently doing a maintenance wash with 0.5% tween every Monday morning, but sometimes the instrument doesn't get used until Thursday/Friday so I'm considering a post run wash in between the days.
-
-
On a schedule like that, I'd do your maintenance wash Monday and then a post-run wash the day you start your sequencing run. If you start the wash when you pull out the cartridge to thaw, you should have plenty of time. -
Recommended washes are outlined in the MiSeq User Guide.
Please find the relevant section attached.Comment
-
NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...Yesterday, 06:46 AM -
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM
Comment