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  • HiSeq 2000 File Sizes

    I was wondering if anyone that is working with a HiSeq 2000 could provide some general file size estimates. I am looking for total file sizes
    - per lane, per flow-cell, or per run
    - intensity files, .bcl files, FASTq files, and/or BAM files
    - for 50, 75, and 100 bp PE and SE runs.

    Thanks,

  • #2
    Attached is the site prep guide. It doesn't give the file sizes you want but does recommend computing specs and archiving space.

    Have you gotten your HiSeq? I have heard that it can take 6 months or more. Our official ship date is over 3 months from placing the order.
    Attached Files

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    • #3
      I have a booklet from a user group meeting that lists:


      Hiseq 200G run
      Image Data 32TB (not tfrd)
      Intensity data 2TB (optionally tfrd)
      BaseCall / Quality score data .25 TB
      Final Alignment output 1.2 TB

      GAIIx 50G run
      Image Data 5.6TB (optionally tfrd)
      Intensity data .5 TB (optionally tfrd)
      BaseCall / Quality score data .06 TB
      Final Alignment output .3 TB

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      • #4
        Our first HiSeq is being validated now

        For 1 36X36 flowcell:

        48G per lane .cif data (Intensities/L00X folders)
        3.3G per lane of _pos.txt files
        200M per lane of .filter files
        6.1G per lane of .bcl data (BaseCalls/L00x folders)
        40G per lane of _qseq.txt files

        Size of BaseCalls folder after BclConverter: 193G
        Total size (Intensities/BaseCalls) 728G

        You don't have to keep the .cif and the .bcl files, but I wanted to test if running Bustard from .cif gave the same results as Bclconverter -- it did almost exactly.
        Christine Brennan
        UM DNA Sequencing Core
        Ann Arbor, MI 48109

        [email protected]

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