We are just preparing our lab for the installation of a HiSeq. We are in an older building so have some concern about the +/- 2C temperature requirements during the length of the run. I am wondering if anyone has any comments on how important this temperature control is.
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We were told by the Illumina engineers that there wasn't a temperature requirement for the HiSeq since the flowcell plate is actively temperature regulated (as opposed to our GA2, which has a peltier unit that comes on and off).
Disclaimer: This is what our lab manager told me.Last edited by Lee Sam; 08-16-2010, 11:17 AM. -
The site prep guide for the HiSeq says that the lab temp must be 19-25C and that the temp cannot vary more than +/-2C during a run.Comment
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I saw this thread when both my FAS and engineer were on site this past Friday and when asked they both said the Hiseq was indeed temperature sensitive.Comment
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Just got off the phone with Illumina. dhinerfeld is spot-on. The room temp must always be 22 +/- 3 and cannot exceed +/-2 during a run. And that does NOT mean it's ok for the temp to swing above 25.Last edited by scrosby; 10-05-2011, 03:16 PM.Comment
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From what our FAS has told us, 68F (20 c) is the sweet spot for the HiSeq's; this isn't as important with respect to the flow cell as it is with respect to the lasers/other components. I personally start getting nervous when I start seeing the ambient temp edging into 69, and if it were to ever hit 71 I'd call facilities.Comment
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