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  • Min Jung Lee
    Member
    • Jan 2014
    • 11

    #1

    Please help me Miseq stopped run with error.

    Hello, everyone.
    I ran miseq and 3hours later had been stopped.
    "Focus mirror sensor not found" error message.
    But! Thumnail images are present 2 cycles.
    Clusters is good by the thumnail images.
    Error message is below.
    "Focus mirror move sensor and/or sensor failed:
    The FPGA reported and error while exacuting command "FOCMRRFOC"
    error message."
    Anyone had experience about this error? And what happend to next?
    Last edited by Min Jung Lee; 03-19-2016, 05:29 PM.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    This is definitely a hardware related error that none of us can help you with. Please contact Illumina tech support. Include a screenshot of the error in your ticket.

    Comment

    • Tim NGS
      Junior Member
      • Mar 2015
      • 4

      #3
      Contact tech support just to be sure.

      Probably an hardware issue. But make sure the instrument is not upgraded to MCS 2.6.0.4 . We used to have similar issues (but can't remember the exact message) a couple of months ago. Loss of focus occured during the run.

      Since our instruments are downgraded to the previous version 2.5.x.x everything seems to work fine again.

      Comment

      • Min Jung Lee
        Member
        • Jan 2014
        • 11

        #4
        Problem solved.

        I supported illumina tech team.
        But error message is poped up again.
        So, I contact illumina engineer in Korea.
        They checked our instrument about focus mirror part.
        And they diagnosed focusing mirror's movements are vey bad, replcement mirror and motor.
        Finally, I ran again same libray.
        Results is very good Q30 is 94.5% and PF is 93.5%!!
        Thank you for everyone.

        Comment

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