Hello,
I want to use MiSeq to metabarcode arthropod samples. I am producing 300 bp amplicons from 130 samples with approximately 600 specimens per sample. This will yield 78,000 300bp amplicons that I want to sequence. Assuming I have the same number of amplicons from each specimen, is it reasonable to expect I will detect most of them?
Because I have 300 bp amplicons, to calculate coverage do I simply divide the number of reads produced in a 2x300 MiSeq run by the number of specimens I want to sequence?
I am still learning the basics of NGS and am unclear on the difference between clusters and reads, among other things.
Thank you for any insights.
I want to use MiSeq to metabarcode arthropod samples. I am producing 300 bp amplicons from 130 samples with approximately 600 specimens per sample. This will yield 78,000 300bp amplicons that I want to sequence. Assuming I have the same number of amplicons from each specimen, is it reasonable to expect I will detect most of them?
Because I have 300 bp amplicons, to calculate coverage do I simply divide the number of reads produced in a 2x300 MiSeq run by the number of specimens I want to sequence?
I am still learning the basics of NGS and am unclear on the difference between clusters and reads, among other things.
Thank you for any insights.
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