Sequence of Nextera transposon

[Mikemike107]

Hi everyone,

it's could be interested for me to obtain the the entire Transposon sequence used in the Nextera® XT DNA Library Prep. Can anybody help me?

Thanks

[pmiguel]

It is Tn5.

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Phillip

[Mikemike107]

Thansk for your response. The entire 5000 bp sequence ?

[pmiguel]

Wait. What do you mean by "transposon"? The protein part is pretty much Tn5, as I understand it, with maybe a couple of minor mutations. The DNA part is not, of course. The 5 kb DNA transposon is replaced with non-connected short sequences containing mainly the cis-regulatory sequence sufficient to cause recognition (binding) by the transposase.
Illumina provides part (or all?) of those sequences in the Illumina Adapter Sequence document:

http://support.illumina.com/downloads/illumina-customer-sequence-letter.html

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Phillip