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  • thsuk1
    Junior Member
    • Jun 2010
    • 7

    #1

    Illumina sequencing data from 1000genomes

    Hi guys,

    I have a question about Illumina sequencing data from
    ftp://ftp-trace.ncbi.nih.gov/1000gen...sequence_read/

    File:ERR004067.filt.fastq.gz 26795 KB
    File:ERR004067_1.filt.fastq.gz 621514 KB
    File:ERR004067_2.filt.fastq.gz 635159 KB

    I know that ERR004067_1 and ERR004067_2 are for paired ends, but I don't know what ERR004067.flit.fastq.gz is.
    I assumed that ERR004067.flit.fastq.gz is a merged file of ERR004067_1.filt.fastq.gz and ERR004067_2.filt.fastq.gz, but size of ERR004067.flit.fastq.gz is much smaller than the others.
    What is ERR004067.flit.fastq.gz?

    thanks.
  • laura
    Senior Member
    • Sep 2008
    • 151

    #2
    The 1000 genomes DCC filters the fastq when they retrieve it from the archive. The filtering criteria are described in this readme

    ftp://ftp.1000genomes.ebi.ac.uk/vol1....sequence_data

    If one half of a paired end passes the filter but the other half fails then the half that passes if put into a fragment file and this is named without the _1 or _2 and should be treated like a single ended fastq file

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