Run already started, but I wrote wrong sample name in sample sheet.
How to change sample name?
Index information is correct.
How to change sample name?
Index information is correct.
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It is not a problem. You can demultiplex sequencing data again with correct names after run has finished. -
How to do demultiplexing? Thank you for your help...Originally posted by nucacidhunter View PostComment
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That depends on the system and machine configuration. Illumina now has 24 hour Tech support ad I would suggest to contact them for tailored support.Comment
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Since sample names are only used to annotate the result files all you would need to do is change the file name once the demultiplexing is done.
If you used an incorrect index/tag sequence then you would need to correct the sample sheet and re-run the demultiplexing using MiSeq reporter or basespace.Comment
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Hey, re-demultiplexing is fairly simple. Here is a PDF explaining it for the MiSeq.
You want to look at page 11 "Requeue Analysis". Basically, you need to open up the Miseq Reporter Software through your browser. Next, go to your analysis folder of this run, and in that folder replace the file that says "SampleSheet.csv" with your new SampleSheet.csv. Make sure you name your file exactly like the old file and the contents are formatted correctly, including the Kit-ID and date etc.
Next you can choose to "Requeue" your sample and run the whole analysis/demultiplexing again.
Let me know if you need further help.Comment
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you can also redemultiplex in basespace by updating the sample sheet (just put the one incorrect sample in the updated sample sheet or all your samples will be duplicated in their projects)Microbial ecologist, running a sequencing core. I have lots of strong opinions on how to survey communities, pretty sure some are even correct.Comment
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