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  • Lien
    Member
    • Dec 2009
    • 47

    #1

    Bst polymerase

    Hi all,

    It might be a stupid question but I was wondering why exactly the Bst polymerase is used during sequencing. Is it because there is no proofreading activity as some websites mention?

    Thanks a lot!
  • ScottC
    Senior Member
    • Jan 2008
    • 244

    #2
    Well, I was told by our Illumina staff that they used Bst because it was the cheapest polymerase they could find! I'm sure there must be other reasons though.

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